Plant leaf Direct PCR kit-UNG (without Sampling Tools) Protocol Direct PCR from Plant Material

Directly use leaf or leaf lysate as a template for PCR amplification, without separate extraction of DNA, fast and high sensitivity. The UNG anti-pollution system has been added to prevent false positives. ◮No time-consuming and expensive DNA purification(Time, labor and cost saved!) ◮Less material ◮Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ◮2 X Mix—reducing sample loading errors and reaction system preparation time ◮Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes ◮High throughput—lysis reaction can be completed in 96-well PCR plate foregene strength
send email to us

Products Details

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Part I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Part II

2× Leaf PCR EasyTM Mix(UNG)
■ No time-consuming and expensive DNA purification ■ Less material ■ Simple-the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding ■ 2 × Mix, reducing sample loading errors and reaction system preparation time ■ Fast-template preparation can be completed in 10 minutes, PCR reaction can be completed in at least 50 minutes ■ High throughput-lysis reaction can be completed in 96-well PCR plateApplication: genetically modified identification, genotyping, etc. Sample: plant leaf Dosage: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method) Detection range: target fragment ≤1kbTwo weeks of PCR Mix detection results under the condition of PCR amplification product contamination.Based on Plant Leaf Direct PCR kit, dUTP is used instead of dTTP, and the UNG enzyme that can degrade the template containing dUTP is added. In this way, the specificity and accuracy of amplification are ensured, and the problem of PCR product contamination that may occur during large-scale genetic testing is prevented.

Previous:20q12/20q13.33 Dual Color Probe

Next:Plant DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol

Micropipette tips l, Rna Extractor, Lyophilizing PCR Reagent, Non-stick spray, Gel electrophoresis ladder, Mold release agent, Rt-PCR Test Reagent Kit, Break Apart probe, China PCR kit and RT-PCR kit, Library Preparation Rna Seq,

Featured Products

Contact us

Please feel free to give your inquiry in the form below We will reply you in 24 hours