Cell Direct RT qPCR Kit---SYBR GREEN I Direct Cell Lysis Cell Ready One-step qRT-PCR Kits

Cat.No.DRT-01011/01012 For cell direct RT-qPCR using 10-1,000,000 cells, and performing RT-qPCR directly from cells without prior RNA purification. Cells are lysed directly to release RNA for RT-qPCR; the high tolerance system makes it unnecessary to purify RNA and directly use cell lysates as RNA templates for RT reactions. Fast and convenient; high sensitivity, strong specificity, and good stability. ◮Simple and effective: with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes. ◮The sample demand is small, as low as 10 cells can be tested. ◮High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates. ◮DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results. ◮Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.
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Products Details

■  Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes. ■ The sample demand is small, as low as 10 cells can be tested. ■ High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates. ■ DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results. ■ Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.Scope of application: cultured cells. - RNA released by sample lysis: only applicable to the RT-qPCR template of this kit. - The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results. 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.  Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.  Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).200×20μl Rxns, 1000×20μl Rxns

Part I

Buffer CL

Foregene Protease Plus II

Buffer ST

Part II

DNA Eraser

5× Direct RT Mix

2× Direct qPCR Mix-SYBR

50× ROX Reference Dye

RNase-Free ddH2O

Instructions

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